CD11b antibody (Biotin)

Catalog No. ABIN120410

The Mouse Monoclonal anti-CD11b antibody has been validated for FACS and IHC (fro). It is suitable to detect CD11b in samples from Rat.

Quick Overview for CD11b antibody (Biotin) (ABIN120410)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD11b antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: OX-42

Product Details anti-CD11b Antibody (Biotin)

Purification: Protein G Chromatography

Immunogen: Rat Peritoneal Macrophages Immunocyte Donor: BALB/c spleen Fusion Partner: NSO/U mouse myeloma cell line

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry. Immunohistochemistry on frozen and paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.02 % NaN3 and EIA grade BSA was added as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: CD11b

Background: CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8 % spleen cells, 44 % bone marrow cells and less than 1 % of thymocytes and is commonly used as a microglial marker in nervous tissue.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor

Gene ID: 10116

UniProt: Q63001

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation